A new panel of NS1 antibodies for easy detection and titration of influenza A virus
Identifieur interne : 002895 ( Main/Exploration ); précédent : 002894; suivant : 002896A new panel of NS1 antibodies for easy detection and titration of influenza A virus
Auteurs : Zhihao Tan [Singapour] ; Sara Akerstrom [Suède] ; Boon Yu Wee [Singapour] ; Sunil K. Lal [Inde] ; Ali Mirazimi [Suède, Singapour] ; Yee-Joo Tan [Singapour]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2010-03.
English descriptors
- Teeft :
- Alternative method, Amino, Amino acid difference, Amino acid residues, Amino acids, Antibodies exhibit binding, Assay, Avian sources, Binding region, Binding site, Binding sites, Biosafety level, Black boxes, Cell biology, Chamber slides, Consensus sequence, Cytopathic effect, Deletion mutants, Denatured forms, Diagnostic assays, Different strains, Easy titration, Effector, Effector domain, Embryonated chicken eggs, Good target, Health database, High degree, Host proteins, Indirect experiments, Infectious disease control, Lipofectamine reagent, Mabs, Mdck, Mdck cell, Mdck cells, Microbiological preparedness, Monitoring virus replication, Monoclonal antibodies, Multiple strains, Mutant, Other hand, Plasmid, Polymorphism, Polymorphism scores, Primary antibody, Protein sequence alignment, Protein sequences, Residue, Rnabinding domain, Room temperature, Schematic representation, Secondary antibody, Sequence alignment, Singapore, Substitution mutants, Supplementary table, Swedish institute, Titration, Uorescent foci units, Viral, Viral infection, Viral replication, Viral titers, Virol, Virus, Virus infection, Virus titers, Western blot analyses, Western blot analysis.
Abstract
The non‐structural protein NS1 of the influenza A virus is a good target for the development of diagnostic assays. In this study, three NS1 monoclonal antibodies (mAbs) were generated by using recombinant NS1 protein of H5N1 virus and found to bind both the native and denatured forms of NS1. Two of the mAbs, 6A4 and 2H6, bind NS1 of three different strains of influenza A virus, namely H1N1, H3N2, and H5N1. Epitope mapping revealed that residues 42–53 of H5N1 NS1 are essential for the interaction with both mAbs. Between the three strains, there is only one amino acid difference in this domain, which is consistent with the observed cross‐reactivities. On the other hand, mAb 1G1 binds to residues 206–215 of H5N1 NS1 and does not bind NS1 of H1N1 or H3N2. Furthermore, all three mAbs detected NS1 proteins expressed in virus infected MDCK cells and indirect immunofluorescence staining with mAbs 6A4 and 2H6 provided an alternative method for viral titer determination. Quantifying the numbers of fluorescent foci units yielded viral titers for three different isolates of H5N1 virus that are highly comparable to that obtained by observing cytopathic effect induced by virus infection. Importantly, this alternative method yields results at 1 day post‐infection while the conventional method using cytopathic effect yields results at 3 days post‐infection. The results showed that this new panel of NS1 antibodies can detect NS1 protein expressed during viral infection and can be used for fast and easy titration of influenza A virus. J. Med. Virol. 82:467–475, 2010. © 2010 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.21709
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001594
- to stream Istex, to step Curation: 001594
- to stream Istex, to step Checkpoint: 000B48
- to stream Main, to step Merge: 002934
- to stream Main, to step Curation: 002895
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A new panel of NS1 antibodies for easy detection and titration of influenza A virus</title><author><name sortKey="Tan, Zhihao" sort="Tan, Zhihao" uniqKey="Tan Z" first="Zhihao" last="Tan">Zhihao Tan</name></author><author><name sortKey="Akerstrom, Sara" sort="Akerstrom, Sara" uniqKey="Akerstrom S" first="Sara" last="Akerstrom">Sara Akerstrom</name></author><author><name sortKey="Wee, Boon Yu" sort="Wee, Boon Yu" uniqKey="Wee B" first="Boon Yu" last="Wee">Boon Yu Wee</name></author><author><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name></author><author><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name></author><author><name sortKey="Tan, Yee Oo" sort="Tan, Yee Oo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C846364E6D1133DDFBE1AB669BC4BB1E036EFF71</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1002/jmv.21709</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-JW9Q4F8B-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001594</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001594</idno><idno type="wicri:Area/Istex/Curation">001594</idno><idno type="wicri:Area/Istex/Checkpoint">000B48</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B48</idno><idno type="wicri:doubleKey">0146-6615:2010:Tan Z:a:new:panel</idno><idno type="wicri:Area/Main/Merge">002934</idno><idno type="wicri:Area/Main/Curation">002895</idno><idno type="wicri:Area/Main/Exploration">002895</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">A new panel of NS1 antibodies for easy detection and titration of influenza A virus</title><author><name sortKey="Tan, Zhihao" sort="Tan, Zhihao" uniqKey="Tan Z" first="Zhihao" last="Tan">Zhihao Tan</name><affiliation wicri:level="1"><country xml:lang="fr">Singapour</country><wicri:regionArea>Collaborative Anti‐Viral Research Group, Institute of Molecular and Cell Biology, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Akerstrom, Sara" sort="Akerstrom, Sara" uniqKey="Akerstrom S" first="Sara" last="Akerstrom">Sara Akerstrom</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, Solna</wicri:regionArea><wicri:noRegion>Solna</wicri:noRegion></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Wee, Boon Yu" sort="Wee, Boon Yu" uniqKey="Wee B" first="Boon Yu" last="Wee">Boon Yu Wee</name><affiliation wicri:level="1"><country xml:lang="fr">Singapour</country><wicri:regionArea>Collaborative Anti‐Viral Research Group, Institute of Molecular and Cell Biology, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, Solna</wicri:regionArea><wicri:noRegion>Solna</wicri:noRegion></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Suède</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Singapour</country><wicri:regionArea>Ali Mirazimi, Centre for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, Nobels Väg 18, SE‐171 82 Solna, Sweden.===Yee‐Joo Tan, Cancer and Developmental Cell Biology Division, Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos Building, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Yee Oo" sort="Tan, Yee Oo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><country xml:lang="fr">Singapour</country><wicri:regionArea>Collaborative Anti‐Viral Research Group, Institute of Molecular and Cell Biology, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Singapour</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Singapour</country><wicri:regionArea>Ali Mirazimi, Centre for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, Nobels Väg 18, SE‐171 82 Solna, Sweden.===Yee‐Joo Tan, Cancer and Developmental Cell Biology Division, Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos Building, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">82</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="467">467</biblScope><biblScope unit="page" to="475">475</biblScope><biblScope unit="page-count">9</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-03">2010-03</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Alternative method</term><term>Amino</term><term>Amino acid difference</term><term>Amino acid residues</term><term>Amino acids</term><term>Antibodies exhibit binding</term><term>Assay</term><term>Avian sources</term><term>Binding region</term><term>Binding site</term><term>Binding sites</term><term>Biosafety level</term><term>Black boxes</term><term>Cell biology</term><term>Chamber slides</term><term>Consensus sequence</term><term>Cytopathic effect</term><term>Deletion mutants</term><term>Denatured forms</term><term>Diagnostic assays</term><term>Different strains</term><term>Easy titration</term><term>Effector</term><term>Effector domain</term><term>Embryonated chicken eggs</term><term>Good target</term><term>Health database</term><term>High degree</term><term>Host proteins</term><term>Indirect experiments</term><term>Infectious disease control</term><term>Lipofectamine reagent</term><term>Mabs</term><term>Mdck</term><term>Mdck cell</term><term>Mdck cells</term><term>Microbiological preparedness</term><term>Monitoring virus replication</term><term>Monoclonal antibodies</term><term>Multiple strains</term><term>Mutant</term><term>Other hand</term><term>Plasmid</term><term>Polymorphism</term><term>Polymorphism scores</term><term>Primary antibody</term><term>Protein sequence alignment</term><term>Protein sequences</term><term>Residue</term><term>Rnabinding domain</term><term>Room temperature</term><term>Schematic representation</term><term>Secondary antibody</term><term>Sequence alignment</term><term>Singapore</term><term>Substitution mutants</term><term>Supplementary table</term><term>Swedish institute</term><term>Titration</term><term>Uorescent foci units</term><term>Viral</term><term>Viral infection</term><term>Viral replication</term><term>Viral titers</term><term>Virol</term><term>Virus</term><term>Virus infection</term><term>Virus titers</term><term>Western blot analyses</term><term>Western blot analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The non‐structural protein NS1 of the influenza A virus is a good target for the development of diagnostic assays. In this study, three NS1 monoclonal antibodies (mAbs) were generated by using recombinant NS1 protein of H5N1 virus and found to bind both the native and denatured forms of NS1. Two of the mAbs, 6A4 and 2H6, bind NS1 of three different strains of influenza A virus, namely H1N1, H3N2, and H5N1. Epitope mapping revealed that residues 42–53 of H5N1 NS1 are essential for the interaction with both mAbs. Between the three strains, there is only one amino acid difference in this domain, which is consistent with the observed cross‐reactivities. On the other hand, mAb 1G1 binds to residues 206–215 of H5N1 NS1 and does not bind NS1 of H1N1 or H3N2. Furthermore, all three mAbs detected NS1 proteins expressed in virus infected MDCK cells and indirect immunofluorescence staining with mAbs 6A4 and 2H6 provided an alternative method for viral titer determination. Quantifying the numbers of fluorescent foci units yielded viral titers for three different isolates of H5N1 virus that are highly comparable to that obtained by observing cytopathic effect induced by virus infection. Importantly, this alternative method yields results at 1 day post‐infection while the conventional method using cytopathic effect yields results at 3 days post‐infection. The results showed that this new panel of NS1 antibodies can detect NS1 protein expressed during viral infection and can be used for fast and easy titration of influenza A virus. J. Med. Virol. 82:467–475, 2010. © 2010 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Inde</li><li>Singapour</li><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Tan, Zhihao" sort="Tan, Zhihao" uniqKey="Tan Z" first="Zhihao" last="Tan">Zhihao Tan</name></noRegion><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name><name sortKey="Tan, Yee Oo" sort="Tan, Yee Oo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Tan, Yee Oo" sort="Tan, Yee Oo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Tan, Yee Oo" sort="Tan, Yee Oo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Tan, Yee Oo" sort="Tan, Yee Oo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Wee, Boon Yu" sort="Wee, Boon Yu" uniqKey="Wee B" first="Boon Yu" last="Wee">Boon Yu Wee</name></country><country name="Suède"><noRegion><name sortKey="Akerstrom, Sara" sort="Akerstrom, Sara" uniqKey="Akerstrom S" first="Sara" last="Akerstrom">Sara Akerstrom</name></noRegion><name sortKey="Akerstrom, Sara" sort="Akerstrom, Sara" uniqKey="Akerstrom S" first="Sara" last="Akerstrom">Sara Akerstrom</name><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name></country><country name="Inde"><noRegion><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002895 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002895 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:C846364E6D1133DDFBE1AB669BC4BB1E036EFF71
|texte= A new panel of NS1 antibodies for easy detection and titration of influenza A virus
}}
| This area was generated with Dilib version V0.6.33. |  |